MSB Vario Cleanup 和 Invisorb Spin Plasmid Mini Kit 试剂盒

Invisorb技术是德国Invitek公司著名的核酸分离和纯化专利技术, 最近该公司又推出了基于第二代Invisorb技术的MSB Vario Cleanup 和 Invisorb Spin Plasmid Mini Kit 试剂盒. MSB Vario Cleanup 试剂盒是超级快速便利的工具用于8bp到30Kb的PCR片断的直接纯化和浓缩, 基于新的缓冲液成分, 纯化过程中PCR片断可以直接和旋转过滤柱(spin filter column or filter tube)的膜结合. 不需要通常的附加洗涤和干燥步骤, DNA可以直接用低盐缓冲液或蒸馏水洗脱出来. 新的专利DNA片断滤膜结合技术不需要使用任何危险的离液序列高的缓冲液. 整个纯化过程大约可以4分钟完成, 纯化后的PCR产物可以即用于下一步的操作,比如酶切, 杂交, 标记, 克隆, 测序和体外杂交.

MSB Vario Cleanup 纯化的PCR产物克隆实验举例

PCR Product Purification                    PCR Product Cloning

Invitek                                        

Supplier Q                            Restriction analysis of 10 randomly selected clones

Comparison of Cloning Results

540 bp PCR products were purified using the MSB® Vario Cleanup (Binding Buffer 2) and the kit from supplier Q

Analysis of the purified PCR products using the Agilent Bioanalyzer

Cloning of 0.1+g purified fragments (stored at –20°C) with the TOPO-TA-Cloning Kit from Invitrogen

317 clones were obtained with fragments purified using the MSB ®Vario Cleanup vs. 14 clones with supplier Q

> 90% Ligation Rate achieved with the MSB® Vario Cleanup

Spin Plasmid Mini Kit Two 提供了超快, 高效和经济的细菌培养物(0.5ml-2ml)质粒DNA分离纯化系统, 该系统把质粒DNA的碱裂解分离步骤和质粒DNA与旋转滤膜(spin filter)高效结合的纯化组合起来, 通过优化的分离方案以及缓冲液,可以获得高产量和高纯度的质粒DNA. 整个过程中, 需要手动处理的时间被减到最少. 该试剂盒分离纯化的质粒DNA可以即用于个中下游运用, 例如PCR, 酶切, 标记, 克隆, 测序等.           

Restriction digests of  pDNA (6Kb pUCpol  from DH5α)              comparison of  pDNA  regarding yield and purity

Lane1  5μl pDNA, undigest                                       20μg pGEM  DNA could be isolated from 2 ml

Lane2  2.5μl EcoRI  digested  pDNA                            over night culture of E. coli. DH5αwith the Inversob

Lane3  5μl pDNA, undigest                                     Spin Plasmid Mini Two

Lane4  2.5μl HindIII  digested  pDNA                            Lane1-3 kit of competitor Q

Lane5  5μl pDNA, undigest                                       Lane4-6 Spin Plasmid Mini Two

Lane6  2.5μl EcoRI  HindIII  digested  Pdna                       Lane7-9 kit of competitor S

 Order information MSB Vario Cleanup

MSB Vario Cleanup (10 purifications)  - (10202309)

MSB Vario Cleanup (50 purifications)  - (10202302)

MSB Vario Cleanup (250 purifications) - (10202303)

MSB Vario Cleanup (500 purifications) - (10202304)

 Order information Spin Plasmid Mini Kit Two

Invisorb Spin Plasmid Mini Kit Two (10 purifications) - (10101409)

Invisorb Spin Plasmid Mini Two (50 purifications) - (10101402)

Invisorb Spin Plasmid Mini Two (250 purifications) - (10101403)

Invisorb Spin Plasmid Mini Two (500 purifications) - (10101404)