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荧光共振能量转移Fluorescence Resonance  Energy Transfer (FRET)

荧光共振能量转移，是指能量从一种受激发的荧光集团(donor 供能),  以非辐射的方式转移到另一种荧光集团(acceptor 受能) 的物理现象，是目前极受到重视的一门高新技术，在生命科学研究中成为一项具有高价值的研究工具，如蛋白质之间相互作用，蛋白质构型改变，核苷酸之间作用，核苷酸与蛋白之间相互作用等. 近年来, FRET技术已发展用于测定蛋白酶, real-time 杂交, PCR 测定以及测定活体细胞内的DNA 相互关系等.

Anaspec 公司是FRET 技术在生物学领域中的前导者,为您提供FRET技术应用的新产品.  

1.      经典FRET染色剂

         1）    EDANS/DABcyl 配对已广泛应用于发展FRET-base 的核酸探针及蛋白酶底物 ;

         2）     MCA/DNP 为另一对供者/ 受者用来发展FRET-base 的核酸探针及蛋白酶底物 

                   与前者比较，此对通常有较短和较弱的荧光信号波长，然而，由于其分子量较小，

                   具有较强的亲和力。

2.      DAbcyl Plus 染色剂

                  DABCYL Plus 为一种很好的水溶性物质， 用来代替DABCYL和 DABSYL                           染色剂

3.      QXLTM Quenchers

                   一系列新型发展的FRET 染色剂

        1)        Simply the best 简单最佳

                ·   强有力的效果 :             与QXLTM配对达到最有效的FRET有效率

                ·   各种反应形式 :             适应于各种不同生物分子蛋白的联结

                ·   完整的quenchers 系列 : 与大部分常用的荧光供体相匹配，并覆盖全部光谱

                ·   增进价值 :                    最佳的效应极其合理的价格

                   ·   QXLTM 670 与 680 : 为Cy5和Cy5-like 荧光染色剂的最有效Quenchers

         2)        HiLyte FluorTM 荧光染色是QXL受者的最佳供者

            3)        一个广泛系列的新型的FRET多肽用于测定蛋白酶

产品介绍

1．             新型测定试剂盒  (FRET 多肽用来测点蛋白酶)

编号

产品名称

大小

规格

 

Capase 测定试剂盒

    

71116

EnzoLyte AFC Caspase Profiling Kit *Fluorimetric*

1 kit

2 plates

71117

EnzoLyte AFC Caspase Substrate Sampler Kit *Fluorimetric*

1 kit

8x100 assays

71114

EnzoLyte AFC Caspase 3 Assay Kit *Fluorimetric*

1 kit

500 assays

71115

EnzoLyte  AFC Caspase 7 Assay Kit *Fluorimetric*

1 kit

500 assays

71120

EnzoLyte  AMC Caspase Profiling Kit *Fluorimetric*

1 kit

2 plates

71121

EnzoLyte  AMC Caspase Substrate Sampler Kit *Fluorimetric*

1 kit

8x100 assays

71118

EnzoLyte AMC Caspase 3 Assay Kit *Fluorimetric*

1 kit

500 assays

71119

EnzoLyte AMC Caspase 7 Assay Kit *Fluorimetric*

1 kit

500 assays

71141

EnzoLyte Rh110 Caspase 3 Assay Kit *Most Sensitive*

1 kit

500 assays

71142

EnzoLyte Rh110 Caspase 7 Assay Kit *Most Sensitive*

1 kit

500 assays

71143

EnzoLyte Rh110 Caspase 3 Screening Kit *Most Sensitive*

1 kit

500 assays

 

MMP 测定试剂盒

    

71136

EnzoLyte 520 MMP Profiling Kit *Fluorimetric*

1 kit

Two 96-well plate

71170

EnzoLyte 520 MMP Substrate Sampler Kit *Fluorimetric*

1 kit

16 x 20 assays

71158

EnzoLyte 520 Generic MMP Assay Kit *Fluorimetric*

1 kit

100 assays

71150

EnzoLyte 520 MMP-1 Assay Kit *Fluorimetric*

1 kit

100 assays

71151

EnzoLyte 520 MMP-2 Assay Kit *Fluorimetric*

1 kit

100 assays

71152

EnzoLyte 520 MMP-3 Assay Kit *Fluorimetric*

1 kit

100 assays

71153

EnzoLyte 520 MMP-7 Assay Kit *Fluorimetric*

1 kit

100 assays

71154

EnzoLyte 520 MMP-8 Assay Kit *Fluorimetric*

1 kit

100 assays

71155

EnzoLyte 520 MMP-9 Assay Kit *Fluorimetric*

1 kit

100 assays

71157

EnzoLyte 520 MMP-12 Assay Kit *Fluorimetric*

1 kit

100 assays

71156

EnzoLyte 520 MMP-13 Assay Kit *Fluorimetric*

1 kit

100 assays

71128

EnzoLyte 490 MMP-1 Assay Kit *Fluorimetric*

1 kit

500 assays

71129

EnzoLyte 490 MMP-2 Assay Kit *Fluorimetric*

1 kit

500 assays

71130

EnzoLyte 490 MMP-3 Assay Kit *Fluorimetric*

1 kit

500 assays

71132

EnzoLyte 490 MMP-7 Assay Kit *Fluorimetric*

1 kit

500 assays

71133

EnzoLyte 490 MMP-8 Assay Kit *Fluorimetric*

1 kit

500 assays

71134

EnzoLyte 490 MMP-9 Assay Kit *Fluorimetric*

1 kit

500 assays

71135

EnzoLyte 490 MMP-13 Assay Kit *Fluorimetric*

1 kit

500 assays

71137

EnzoLyte 490 MMP-12 Assay Kit *Fluorimetric*

1 kit

500 assays

 

Secretase 测定试剂盒

    

71144

EnzoLyte 520 £]-Secretase Assay Kit

1 kit

100 assays

 

病毒蛋白酶测定试剂盒

    

71127

EnzoLyte 490 HIV Protease Assay Kit *Fluorimetric*

1 kit

500 assays

71147

EnzoLyte 520 HIV Protease Assay Kit *Fluorimetric*

1 kit

100 assays

71126

EnzoLyte 490 HCV Protease Assay Kit *Fluorimetric*

1 kit

500 assays

71145

EnzoLyte 520 HCV Protease Assay Kit *Fluorimetric*

1 kit

100 assays

71146

EnzoLyte 620 HCV Protease Assay Kit *Fluorimetric*

1 kit

100 assays

 

基因蛋白酶测定试剂盒

    

71124

EnzoLyte Green Protease Assay Kit *Fluorimetric*

1 kit

500 assays

71140

EnzoLyte Red Protease Assay Kit *Fluorimetric*

1 kit

500 assays

 

磷酸化酶测定试剂盒

    

71100

EnzoLyte FDP Protein Phosphatase Assay Kit *Fluorimetric*

1 kit

500 assays

71104

EnzoLyte MFP Protein Phosphatase Assay Kit *Fluorimetric*

1 kit

500 assays

71105

EnzoLyte pNPP Protein Phosphatase Assay Kit *Colorimetric*

1 kit

500 assays

71108

EnzoLyte FDP Secreted Alkaline Phosphatase Reporter Gene Assay Kit *Fluorimetric*

1 kit

500 assays

71233

EnzoLyte pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit *Colorimetric*

1 kit

500 assays

71109

EnzoLyte FDP Alkaline Phosphatase Assay Kit *Fluorimetric*

1 kit

500 assays

71230

EnzoLyte pNPP Alkaline Phosphatase Assay Kit *Colorimetric*

1 kit

500 assays

71103

EnzoLyte MG Phosphate Assay Kit *Colorimetric*

1 kit

1000 assays

71231

EnzoLyte MFP Acid Phosphatase Assay Kit *Fluorimetric*

1 kit

500 assays

71101

EnzoLyte FDP Alkaline Phosphatase ELISA Assay Kit *Fluorimetric*

1 kit

500 assays

71232

EnzoLyte pNPP Alkaline Phosphatase ELISA Assay Kit *Colorimetric*

1 kit

500 assays

71110

EnzoLyte ADHP Peroxidase ELISA Assay Kit *Fluorimetric*

1 kit

500 assays

 

辣根过氧化酶测定试剂盒 

   

71111

EnzoLyte ADHP Peroxidase Assay Kit *Fluorimetric*

1 kit

500 assays

 

氢化辣根过氧化酶测定试剂盒 

   

71112

EnzoLyte ADHP Hydrogen Peroxide Assay Kit *Fluorimetric*

1 kit

500 assays

 

细胞增值与细胞毒性测定试剂盒

    

71300

DHL Cell Viability and Proliferation Assay Kit *Fluorimetric*

1 kit

1000 assays

71302

DHL Cell Cytotoxicity Assay Kit *Fluorimetric*

1 kit

500 assays

71405

DHL Express Cell Counting Kit *Fluorimetric*

1 kit

500 assays

 

蛋白标记试剂盒

    

71000

AnaTag AMCA-X Protein Labeling Kit *Ultra Convenient*

1 kit

5 reactions (5 x 5 mg protein)

71001

AnaTag 5-FAM Protein Labeling Kit

1 kit

5 reactions (5 x 5 mg protein)

71002

AnaTag 5-TAMRA Protein Labeling Kit *Ultra Convenient*

1 kit

5 reactions (5 x 5 mg protein)

71005

AnaTag 5-ROX Protein Labeling Kit *Ultra Convenient*

1 kit

5 reactions (5 x 5 mg protein)

71006

AnaTag TR Protein Labeling Kit

1 kit

5 reactions (5 x 5 mg protein)

71007

AnaTag HiLyte Fluor? 555 Protein Labeling Kit *Ultra Convenient*

1 kit

5 reactions (5 x 100 µg protein)

71008

AnaTag HiLyte Fluor 647 Protein Labeling Kit

1 kit

5 reactions (5 x 5 mg protein)

71009

AnaTag AP Protein Labeling Kit *Ultra Convenient*

1 kit

1 reaction (1 x 1 mg protein)

71010

AnaTag HRP Protein Labeling Kit

1 kit

1 reaction (1 x 1 mg protein)

71011

AnaTag APC Protein Labeling Kit

1 kit

1 reaction (1x1 mg protein)

71012

AnaTag BPE Protein Labeling Kit

1 kit

1 reaction (1x1 mg protein)

71013

AnaTag RPE Protein Labeling Kit

1 kit

1 reaction (1x1 mg protein)

2.            经典FRET 染色剂

编号

产品名称

规格

23486

Boc-Asp(EDANS)-OH

1 g

23488

Boc-Glu(EDANS)-OH

1 g

23490

Boc-Lys(DABCYL)-OH

1 g

81800

DABCYL acid

1 g

81801

DABCYL acid, SE

100 mg

81819

DABCYL C2 amine

100 mg

81802

DABCYL C2 maleimide

25 mg

81820

DABCYL hydrazide

100 mg

81808

DABSYL-L-alanine

25 mg

81806

DABSYL chloride

1 g

81809

DABSYL-glycine

25 mg

81807

DABSYL hydrazine

100 mg

81810

DABSYL-L-leucine

25 mg

81811

DABSYL-L-methionine

25 mg

81812

DABSYL-L-proline

25 mg

81813

DABSYL-L-tryptophan

25 mg

81814

DABSYL-L-valine

25 mg

81227

DNP-X acid

100 mg

81228

DNP-X acid, SE

25 mg

81821

DNP C2 amine

100 mg

81822

DNP C2 maleimide

25 mg

23887

EDANS, acid

1 g

81535

EDANS, sodium salt

1 g

23492

Fmoc-Asp(EDANS)-OH

1 g

23494

Fmoc-Glu(EDANS)-OH

1 g

23496

Fmoc-Lys(DABCYL)-OH

1 g

23858

Fmoc-Lys(DABCYL) AnaResin

500 mg

3.      DAbcyl Plus 染色剂

编号

产品名称

规格

81803

DABCYL Plus acid

100 mg

81804

DABCYL Plus acid, SE

25 mg

81823

DABCYL Plus C2 amine

10 mg

81805

DABCYL Plus C2 maleimide

10 mg

81824

DABCYL Plus hydrazide

10 mg

4.                QXLTM Quenchers

编号

产品名称

规格

81825

QXLTM 490 acid

100 mg

81826

QXLTM  490 acid, SE

25 mg

81828

QXLTM  490 C2 amine

10 mg

81846

QXLTM  490 hydrazide

5 mg

81830

QXLTM  520 acid

100 mg

81831

QXLTM  520 acid, SE

25 mg

81832

QXLTM  520 C2 amine

10 mg

81847

QXLTM  520 hydrazide

5 mg

81833

QXLTM  520 C2 maleimide

10 mg

81835

QXLTM  570 acid

25 mg

81836

QXLTM  570 acid, SE

10 mg

81837

QXLTM  570 C2 amine

5 mg

81848

QXLTM  570 hydrazide

5 mg

81838

QXLTM 570 C2 maleimide

5 mg

81815

QXLTM 610 acid

100 mg

81816

QXLTM 610 acid, SE

25 mg

81818

QXLTM 610 C2 amine

25 mg

81849

QXLTM 610 hydrazide

10 mg

81817

QXLTM 610 vinyl sulfone *Thiol-Reactive*

25 mg

81840

QXLTM 670 acid

10 mg

81841

QXLTM 670 acid, SE

5 mg

81842

QXLTM 670 C2 amine

5 mg

81850

QXLTM 670 hydrazide

5 mg

81843

QXLTM 670 C2 maleimide

5 mg

81851

QXLTM 680 acid, SE

5 mg

81852

QXLTM 680 C2 amine

1 mg

81853

QXLTM 680 hydrazide

1 mg

81854

QXLTM 680 C2 maleimide

1 mg

81855

QXLTM 680 acid

10 mg
 
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